γ-aminobutyric acid is a kind of amino acid widely used in medicine, health care products, feed and other fields, referred to as GABA, with its development in recent years, we found that in functional food, γ-aminobutyric acid has a great prospect for development.
We can obtain γ-aminobutyric acid by chemical synthesis method, plant enrichment method, microbial method and other methods. In the chemical synthesis method there are many kinds of raw materials can be used to prepare γ-aminobutyric acid, such as potassium phthalimide and 4-chloroprene as raw materials, reaction at 180℃, the product obtained with concentrated sulfuric acid hydrolysis, γ-aminobutyric acid can be obtained; Pyrrolidone can also be used as raw material, after ammonium bicarbonate and calcium hydroxide hydrolysis of γ-aminobutyric acid; Also can butyric acid and ammonia water as raw materials, in the γ-ray light reaction, get γ-aminobutyric acid.
Both column separation and preparation method and solvent extraction method belong to plant enrichment method. Among them, column separation and preparation method uses the principle of different distribution coefficients of components in different mixtures of solid and liquid phases to carry out elution separation and other subsequent processing. In the solvent extraction method, the extraction agent is water or alcohol, according to the plant solubility in water or alcohol and distribution system, γ-aminobutyric acid is mentioned in water or alcohol. After repeated filtration and purification, gamma-aminobutyric acid can be extracted from plants.
Microbial fermentation method is a method with strict requirements on environment and equipment, which can obtain γ-aminobutyric acid in the process of growth and reproduction of excellent stable, non-toxic and harmless bacteria.