What are the steps required to prepare 3,4-dihydroxybenzoic acid?

3,4-dihydroxybenzoic acid is a metabolite of polyphenols, belonging to phenolic acid, which is widely used in medicine, health care products, cosmetics and other industries.

There are several ways to extract 3,4-dihydroxybenzoic acid, for example, protocatechuic acid from the leaves of the plant lilac. The method is divided into two parts: pre-treatment and extraction of raw materials. This requires drying of lilac, removing seeds and other sundries after optimization, and crushing the remaining parts to obtain fine powder.

The fine powder obtained above is crushed to 70 mesh sieve, and 2 grams of it is used as raw material. Under certain technological conditions, anhydrous ethanol is used as the extraction agent to extract the extracted solution. The extracted solution is filtered, and the residue is removed to recover the ethanol, and the crude 3,4-dihydroxybenzoic acid can be obtained.

If you want to get the fine 3,4-dihydroxybenzoic acid, you need to do some work by adding 50ml of ethanol and 3ml of 0.1mol/L hydrochloric acid to the rough 3,4-dihydroxybenzoic acid and shaking them to dissolve together. And then put into a clean separation funnel, and the use of 0.1mol/L sodium hydroxide solution for extraction, operation three times, each extraction 50ml, at the same time, the use of 10ml ether after extraction of sodium hydroxide solution for washing, ether solution combined, adjust the PH value to 6, evaporation and drying, The refined 3,4-dihydroxybenzoic acid was obtained as white powder crystal.

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