How to prepare L-tyrosine from casein?

L-tyrosine is a white powder, soluble in dilute acid and alkali, insoluble in anhydrous ethanol, ether and acetone, it has no odor, but has a bitter taste. At present,L-tyrosine is mainly used in food, feed, medicine, daily chemical and other fields as a nutritional enhancer, moisturizing agent, etc., can also be used in biochemical research.

In the field of biochemical research, L-tyrosine can not only be used as a standard for the determination of nitrogen in amino acids, but also used for colorimetric quantitative analysis of Milon reaction, and can also be used to prepare tissue culture medium, as the precursor of amino acids for dopamine and catecholamine.

Using casein or spun silk protein, casein or pig blood meal, ox horn hoof, silk and other raw materials can get L – tyrosine, therefore, get a lot of methods L – tyrosine, for example, using casein as raw materials, in hydrochloric acid reflux after hours of filtration, concentration, alkali neutralization and activated carbon treatment, L – tyrosine crystal. Casein or silk and other proteins can also be used as raw materials. The product obtained after acid hydrolysis is then neutralized. After precipitation separation, it is dissolved in dilute ammonia and then neutralized with acetic acid to pH value 5. L-tyrosine can be obtained from pig blood meal, cow horn hoof or silk after acid hydrolysis.

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